The purpose of this study is to investigate distribution of the HLA antigens among Korean population and to compare the results with the previous publications. The subjects for this study consisted of 85 normal healthy persons and 84 patients with various diseases. The method of detection of HLA antigens was the microtoxicity test deviced by Terasaki, the lymphocytes being separated from peripheral blood by density-gradient centrifugation in the Ficoll-Hypaque solution. The standard HLA antiserum used in this study was the T-series antiserum provided by the Behringwerke Company, West Germany. The antiserum included HLA-A 1, 2, 3, 9, 10, 11, and HLA-B 5, 7, 8, 12. The positive and negative control sera were also used.
The observed data are summarized as follows:
1. The detection rate of the HLA antigens was essentially the same among normal healthy persons, and patients with various diseases.
2. In the normal healthy group, HLA-A2 and A3 were most frequently detected in the first segregation series, and HLA-B 12 and B 7 in the second segregation series, respectively.
3. In the group with various -diseases, the most frequent HLA antigens were HLA-A2 and A10 in the first segregation series, and HLA-B5 and B 8 in the second segregation series, respectively.
4. The HLA frequencies among the total of 169 subjects studied in this series were essentially the same as the normal healthy group.
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